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. 2016 May 5;4(2):15. doi: 10.3390/vaccines4020015

Figure 2.

Figure 2

Production of antibody to CCMV, S9 peptide, and GBS by mice immunized with different immunogens. Groups of four mice were immunized with ssCCMV VLPs, ssCCMV VLPs conjugated to S9 peptide, subE (monomeric capsid) conjugated to S9 peptide, CPMV VLPs conjugated to S9 peptide, and KLH conjugated to S9 peptide. Mice were immunized either with Freund’s adjuvant, or in the absence of adjuvant. The results of individual mice are shown. Prebleeds are in open bars, sera obtained following immunization in solid bars. ELISA plates were coated with the indicated antigens. Sera were tested at 1:1000 dilution for CCMV, 1:5000 for S9 peptide, and 1:200 for GBS. Binding was detected with alkaline phosphatase conjugated anti-mouse IgG (H + L). Antibodies of all isotypes are detected because of the L chain specificity of the conjugated antiserum.