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Antibody responses in a second experiment. Groups of six mice were immunized with CCMV, CCMV-S9, CPMV-S9, and KLH-S9 with no adjuvant. In panel A, prebleed (open bar) and post-immune serum (filled bar) were tested for binding to CCMV, S9 peptide, and GBS and detected with alkaline phosphatase–conjugated anti-mouse IgG (H + L). In panel B, the sera are tested for IgG subclass of antibody to the S9 peptide.
