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Cytokine production by lymphocytes from VLP- or carrier-immunized mice. Mice immunized with CCMV-S9 or KLH-S9 were boosted and, four days later, sacrificed. Lymphocytes from mesenteric lymph nodes and spleens were pooled from mice in each group and splenocytes isolated. The cells were incubated with the indicated antigens and cytokines in the cell supernatants measured on day 3 (IFN-γ) or day 5 (IL-4 and IL-10) using cytokine capture ELISAs.
