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. 2016 Jul 4;6:29310. doi: 10.1038/srep29310

Figure 1. PCR amplification of IL-8 gene, identification of recombinant plasmid pMD19-T-IL-8 and sequence analysis of IL-8 amino acid sequence.

Figure 1

(A) PCR amplification of IL-8 gene. M1: DNA marker (DL2000); lane 1: PCR product of IL-8 gene (336 bp). (B) Identification of recombinant plasmid pMD19-T- IL-8 with PCR and enzyme digestion. M1: DNA marker (DL2000); lane 1: PCR product of plasmid pMD19-T-IL-8; lane 2: digestion of plasmid with EcoR I and Xho I; lane 3: digestion of plasmid with XhoI; M2: DNA marker (DL10000). (C) Amino acid sequence analysis of IL-8 sequence with other channel catfish IL-8 s. Dotted box presented signal peptides, shadow regions presented the conserved Chemokine_CXC domain. The ELR motif, CXC motif and GPH motif were indicated by blue, red and black box, respectively.