Table 1. Primers used for cloning, expression and qRT-PCR of genes in this paper.
| Primers | Sequences (5′→3′)a | Target gene |
|---|---|---|
| IL-8-F1 | CGGAATTCATGAAGGCTGCAACT (EcoRI) | IL-8 |
| IL-8-R1 | CCCTCGAGTCAGTTTTGCTGTTTG (XhoI) | |
| IL-8-F2 | CGGAATTCGGAGAAGGAAAAGCAGAG (EcoRI) | tIL-8 |
| IL-8-R2 | CCCTCGAGTCAGTTTTGCTGTTTGATCT (XhoI) | |
| ACTB-F | CCCATCTATGAGGGTTATGCTCTG | ACTB |
| ACTB-R | GCTCGGTCAGGATCTTCATCAG | |
| IL-1β-F | GCCATGTTGCTAATGTTGTAATCG | IL-1β |
| IL-1β-R | TGTCTTGCAGGCTGTAACTCTTG | |
| TNF-α-F | CGCACAACAAACCAGACGAGAC | TNF-α |
| TNF-α-R | ACCACTGCATAGATACGCTCGAA | |
| CXCL10-F | ACAGGCCAGGACCAGTGTAAGG | CXCL10 |
| CXCL10-R | CAAGTTGCACTCGCAGGATGAA | |
| MHC Iα-F | GGTATCATCGTTGGTGTAGCCG | MHC Iα |
| MHC Iα-R | GGACAGGTTTGAAGCCAGAGTT | |
| MHC IIβ-F | CGGGAAGGAGATTAAAGGAGGT | MHC IIβ |
| MHC IIβ-R | GTTTGGTGAAGCTGGCGTGT | |
| CD4-L2-F | GCAGGGCACGGATAGATGGA | CD4-L2 |
| CD4-L2-R | TGGGTTCGCAGAGGCTGATAC | |
| CD8α-F | CCGACAGTGCCTACGACTAAAGC | CD8α |
| CD8α-R | CCAGCAGCCAAAGGAATGAAG | |
| IFN-γ-F | TGCACGAAGTGAAAGACCAAA | IFN-γ |
| IFN-γ-R | TTAAGGTCCAGCAGCTCAGTGA |
aUnderlined nucleotides are restriction sites of the enzymes indicated in the brackets at the ends.