Table 1.
Metabolites detected in 10 μl cell culture medium measured by GC-MS
| Metabolite | DMEM | DMEM and serum | HPDE cells | PDAC cells |
|---|---|---|---|---|
| Lactate | − | + | +++ | +++ |
| Pyruvate | + | + | +++ | +++ |
| Succinate | − | + | − | + |
| 2-oxoglutarate | + | + | ++ | − |
| Malate | − | ++ | + | ++ |
| Citrate | − | +++ | + | ++ |
| Glycerol | + | +++ | + | ++ |
| Alanine | − | + | ++ | ++ |
| Aspartate | − | + | + | + |
| Glutamate | − | + | ++ | ++ |
| Glutamine | + | ++ | + | + |
| Glycine | + | + | + | ++ |
| Serine | + | + | + | + |
| Proline | − | + | + | ++ |
Concentrations were scored qualitatively with “+”, “++” and “+++” to show relative quantity. Metabolites with peak area less than 5 % of the largest peak are marked with “−”