Fig. 3.

Boxplot of chromatin structure and transcription level in the promoter region of SmPoMuc3.1 (r1r2). Two technical replicates for each biological replicate (n). a ChIP H3K9me3 to H3K9ac ratio at the promoter of SmPoMuc 3.1(r1r2). b Relative transcription of SmPoMuc 3.1(r1r2) compared to alpha-tubulin measured by RT-qPCR. ***indicate statistical significant differences according to ANOVA followed by Dunnett’s post hoc test to F0 SmBRE that was defined as reference (ANOVA F _2,8 = 13.128; p = 0.003 for transcription and F _2,14=7.26; p = 0.007 for H3K9me3/ac ChIP ratio). Only SmGH2 is significantly different in chromatin structure and transcription level from the reference. Genotype SmGH2 of the F3 is similar (not different) to reference genotype F0 SmBre in terms of chromatin structure and transcription. For additional pairwise comparison we used Students T-Test. We found for H3K9me3/ac ratio (a) t = 10.045, 8 df, p < 0.0001, i.e. there is statistically significant difference between SmGH2 and F3-SmGH2. For transcription (b) the difference between SmGH2 and F3-SmGH2 is statistically not significant (t = 1.514; 5 df; p = 0.1904) since variance is strong. Nevertheless, there is a clear shift of transcription in F3-SmGH2 towards the lower level of transcription observed in SmBRE