Figure 5.

RNA quantity and quality from tissue fixed in different fixative solutions. (A) RNA quantity extracted from each specimen was measured by the Nanodrop spectrophotometer. RNA extraction yield is expressed as the mean of three replicates (mean ± standard deviation [SD]). (B) Representative data are presented as an electropherogram. To compare the quality of RNA extracted under each condition, we used an electropherogram to overlay all seven different conditions. (C) RNA integrity is presented as paraffin-embedded RNA metric (PERM). Abbreviations: E, 70% ethanol; BE70, buffered ethanol 70%; NBF, neutral-buffered formalin; FF, fresh frozen mouse kidney (positive control).