Figure 7.

DNA quantity and quality from tissue fixed in different fixative solutions. (A) Quantity of RNA extracted from each specimen was measured with the Nanodrop spectrophotometer. DNA extraction yield is expressed as the mean of three replicates (mean ± SD). (B) DNA quality was assessed using the BioScore™ Screening and Amplification kit. We amplified approximately 10 µg DNA using a 100-ng DNA template from buffered ethanol 70% (BE70). (C) Mean cycle threshold (Ct) values of the housekeeping gene hypoxanthine-guanine phosphoribosyltransferase (HPRT) were determined in kidney under different fixative conditions. Gene expression levels are shown as box plots. The values are the mean polymerase chain reaction Ct-values. Bars indicate standard deviations. Abbreviations: E, 70% ethanol; NBF, neutral-buffered formalin; FF, fresh frozen mouse kidney (positive control). *p < 0.05, **p < 0.01, ***p < 0.001.