Fig. 2.

(A) Measurement of serum osteocalcin concentration from 6- and 14-week-old WT and TgCAT mice. Statistical significance was determined by one-way analysis of variance followed by Student-Newman-Keuls post hoc analysis; values with different letter subscripts are statistically different from each other (P < 0.05). (B) Total number of mature osteoclasts from WT and TgCAT bone marrow cells as identified by TRAP staining after culture in media containing 15 ng of RANKL and 20 nM 1,25(OH)₂D₃ for 10 days. Statistical differences were determined by Student’s t test; values with different letter subscripts are statistically different from each other (P < 0.05). OC, osteocalcin.