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. Author manuscript; available in PMC: 2016 Jul 4.
Published in final edited form as: Curr Drug Metab. 2014;15(7):743–756. doi: 10.2174/1389200216666141208151524

Fig. (1).

Fig. (1)

Simplified schematic of using protein engineering to redesign an enzyme’s substrate specificity highlighting knowledge-based mutagenesis, computational protein design, and directed evolution.