Table 4.

Genotype laboratory methods for high-quality genotype–genotype studies (n = 6).

Study	Year	Index method	Reference method(s)	Polymorphisms tested	Ref.
Chowdhury	2007	Microchip RFLP	Conventional RFLP and AS-PCR; integrated microchip PCR and AS-PCR; TaqMan SNP genotyping	TPMT*2, TPMT*3a, TPMT*3b, TPMT*3c	[71]
Kim	2013	AS-PCR	PCR	TPMT*2, TPMT*3a, TPMT*3b, TPMT*3c	[75]
Lu	2005	APEX	ARMS-PCR; PCR-RFLP	TPMT*3b, TPMT*3c, TPMT*6	[76]
Ma	2003	PCR + DHPLC	PCR + RFLP; PCR + SNaPshot sequencing with direct DNA sequencing; AS-PCR	TPMT*2, TPMT*3a, TPMT*3b, TPMT*3c, TPMT*3d	[70]
Roman	2012	LightSNiP	Traditional PCR and Sanger sequencing	TPMT*2, TPMT*3b, TPMT*3c	[15]
Schaeffeler	2008	MALDI-TOF MS	DHPLC	TPMT*2, TPMT*3a, TPMT*3c, TPMT*9, TPMT*11, TPMT*16, TPMT*17, TPMT*18, TPMT*20, TPMT*22	[74]

APEX: Arrayed primer extension technology; AS-PCR: Allele-specific PCR; DHPLC: Denaturing high performance liquid chromatography; MS: Mass spectrometry; TMPT: Thiopurine s-methyltransferase.