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. Author manuscript; available in PMC: 2016 Jul 4.
Published in final edited form as: Stem Cells. 2016 Mar 4;34(7):1922–1933. doi: 10.1002/stem.2339

Figure 6. Analysis of CADM1 for a Function Role in EPC Migration in Response to TNFα.

Figure 6

(A) PCR Analysis of CADM1 Expression in Response to Inhibitors. (1) CADM1 gene expression was measured in response to TNFα in the presence of a synthetic peptide inhibitor of NF-kB or the scrambled control. In presence of the scrambled control TNFα induced up regulation of CADM1 (P < 0.05) whereas in presence of the inhibitor this response was abolished. (2) In the presence of scrambled siRNA, TNFα was able to increase gene expression of CADM1 (P < 0.05) whereas in the presence of targeting siRNA this phenotype was abolished (n = 16 per group).

(B) EPC Migration with CADM1 Knockdown. Prior to TNFα administration, EPCs were transfected with a CADM1 siRNA or a scrambled control. The TNFα induced migratory phenotype was still observed in the presence of the scrambled inhibitor at 2 hour and 14 hours (P < 0.05) but the phenotype was abolished at 2 hours and 14 hours when the targeted siRNA was transfected (n = 16 per group).