Table 2.
Comparison of stoichiometric and kinetic values of the anaerobic conversions determined in this study and previous studies with enriched PAO and GAO cultures
| References | Sequencing batch reactor (SBR) Batch reactor (BR), |
Organisms PAO II, PAO I, PAO, GAO | SRT (days) |
HRT (h) |
pH | Influent [Ca2+] (mg l−1) |
VSS/ TSS (mg mg−1) |
PHV/PHB (C-mol C-mol−1) |
PHV/HAc (C-mol C-mol−1) |
PHB/HAc (C-mol C-mol−1) |
P/HAc (P-mol C-mol−1) |
Gly/HAc (C-mol C-mol−1) |
C-mol (C-mol h)−1 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| This study | SBR | PAO II and GAO | 8 | 12 | 7 ± 0.1 | 3.8 | 0.96 | 0.37 | 0.54 | 1.45 | 0.03 | 1.28 | 0.14 |
| Zeng et al. (2003) | SBR | GAO | 6.6 | 8 | 7 ± 0.1 | 6.8 | 0.97 | 0.38 | 0.52 | 1.39 | NA | 1.20 | 0.16–0.18 |
| Lopez-Vazquez et al. (2007) | SBR | GAO | 10 | 12 | 7 ± 0.1 | 3.8 | 0.9 | 0.34 | 0.69 | 1.28 | 0.01 | 1.20 | 0.20 |
| Welles et al. (2015) | SBR | PAO II | 8 | 12 | 7 ± 0.05 | 3.8 | 0.75 | 0.19 | 0.23 | 1.24 | 0.22 | 0.96 | 0.15 |
| BR | PAO II | NA | NA | 7 ± 0.1 | 3.8 | 0.93 | 0.27 | 0.32 | 1.19 | 0.01 | 0.98 | 0.08 | |
| SBR | PAO I | 8 | 12 | 7 ± 0.1 | 3.8 | 0.58 | 0.07 | 0.09 | 1.27 | 0.64 | 0.29 | 0.18 | |
| BR | PAO I | NA | NA | 7 ± 0.1 | 3.8 | 0.95 | 0.33 | 0.37 | 1.09 | 0.02 | 1.28 | 0.02 | |
| Zhou et al. (2008) | SBR | PAO | 8 | 24 | 7.0–8.0 | 1.3 | 0.6 | 0.06 | 0.07 | 1.18 | 0.62 | 0.46 | NA |
| BR | PAO | NA | NA | 7.5 ± 0.01 | 1.3 | NA | 0.37 | 0.46 | 1.24 | 0.06 | 1.03 | 0.07 | |
| Acevedo et al. (2012) | SBR | PAO I | 8 | 12 | 7.0–8.9 | 10 | 0.45 | 0.04 | 0.05 | 1.31 | 0.7 | 0.38 | NA |
| SBR | PAO I, II | 8 | 12 | 7.0–8.9 | 10 | 0.92 | 0.16 | 0.28 | 1.74 | 0.08 | 1.08 | NA | |
| Tian et al. (2013) | SBR | PAO I | 16 | 12 | 7 ± 0.1 | 3.8 | NA | 0.1 | 0.13 | 1.31 | 0.56 | 0.55 | NA |
| Welles et al. 2014 | SBR | GAO | 8 | 12 | 7.0 | 3.8 | 0.97 | NA | NA | NA | 0.012 | 1.2 | 0.15 |
| Filipe et al. (2001) | SBR | ?b | 7 | 12 | 6.8–7.1 | 3.8 | NA | 0.31 | 0.38 | 1.26 | NA | 0.83 | 0.24 |
| Sudiana et al. (1999) Reactor ALa | SBR | ?c | NA | NA | 6.8–7.2 | NA | NA | 0.24 | 0.4 | 1.7 | 0.02 | 1.30 | 0.06–0.08 |
| Liu et al. (1997) a | SBR | ? | 8 | 6 | 7 ± 0.1 | 7.6 | NA | NA | NA | NA | 0.02 | 1.37 | 0.04 |
| Schuler and Jenkins (2003) a | SBR | ? | 4 | 12 | 7.15–7.25 | 16 | NA | NA | NA | NA | 0.11 | 1.19 | 0.03 |
specific maximum anaerobic HAc-uptake rate
NA not applicable
aCalculated assuming that the VSS fully comprised of active biomass
bDGGE banding patterns indicated that 75 % of the population belonged to γ-proteobacteria, while with FISH analysis, using specific probes developed to target the dominant γ-proteobacteria in the DGGE banding pattern, showed that 35 % of the population stained positive for these γ-proteobacteria
cFISH analysis revealed that β-proteobacteria were dominant, comprising about one-third of the sludge