FIGURE 6.

Effect of amplicon arm size on transformation. (A) Gel analysis of six amplicons of different sizes derived from the same genomic template (MI009). L, DNA ladder Gene Ruler 1kb (GenScript). (B) Transformation as a function of amplicon arm sizes. S. mitis type strain pre-culture at OD₆₀₀ 0.5 was diluted 1:100 in C+Y_{Y B} and grown until OD₆₀₀ 0.04 at 37°C in 5% CO₂. Following, 200 μL aliquots were exposed to 300 nM of CSP, 150 ng mL^-1 donor DNA amplicon (aRJ20, aRJ22, aRJ23, aRJ24, aRJ25, and aRJ26) and further incubated in closed tubes for 4 h at 37°C in air atmosphere. The % of CFU containing Erm^R transformants was determined by plating on blood plates with and without Erm. The results are averages from three replicates and representative of three independent experiments. Error bars represent standard error of the mean.