FIGURE 7.

Transformation as a function of different protocols and donor DNA types. Following the optimized protocol, S. mitis type strain pre-culture at OD₆₀₀ 0.5 was diluted 1:100 in C+Y_{Y B} and grown until OD₆₀₀ 0.04 at 37°C in 5% CO₂. Then, 200 μL aliquots were exposed to 300 nM of CSP and 150 ng mL^-1 amplicon (aRJ20) or 1 μg replicative plasmid pVA838. Cells were further incubated in closed tubes for 3 h at 37°C air atmosphere. The % of CFU containing Erm^R transformants was determined by plating on blood plates with and without Erm. The results are averages from two independent experiments. Bars represent standard error of the mean. Differences in efficiency between media were analyzed by Student’s T-test (^∗p < 0.05).