FIGURE 3.

Birth-dating of striatal neurons after quinolinic acid lesion to assess the effect of GDNF and Meteorin on these populations. (A) Quantification of BrdU⁺ cell density across treatment groups revealed no significant change in newborn cells in the striatum. (B) Quantification of BrdU⁺ cells co-expressing NeuN across treatment groups normalized to BrdU⁺ cell density revealed lvGDNF increased the number of new neurons in the ipsilateral striatum, with lvMeteorin having no effect compared to lvGFP controls. (C) Quantification of BrdU⁺/NeuN⁺/CalR⁺ cells normalized to BrdU⁺ cell density identified lvGDNF treatment trended toward an increase in this population. (D) CalR⁺ cells normalized to total BrdU⁺/NeuN⁺ cells indicated differentiation into this phenotype is altered across all newborn neurons. Overview image of BrdU/NeuN/CalR staining (E) and representative orthogonal z-stack confocal images of immuno-labeled (F) BrdU⁺/NeuN⁺/Darpp32^- neurons and (G) BrdU⁺/NeuN⁺/CalR⁺ interneurons (arrow). (H) NeuN negative interneurons were also observed as a primary subset of BrdU⁺/Calretinin⁺ cells (arrows). Data represents mean ± SEM. lvGFP n = 7; lvMeteorin n = 6; lvGDNF n = 5. Scale bars: 100 μm. cc, corpus callosum; lv, lateral ventricle; ac, anterior commissure; CalR, Calretinin.