Figure 9. Gbp4-mediated S. Typhimurium clearance in vivo is associated to the inflammasome-dependent production of prostaglandin D2.

(a–e) Zebrafish one-cell embryos were injected with standard control (std) or Gbp4 MOs, treated by immersion with vehicle alone (methyl acetate), 10 μM 16, 16-dimethyl-prostaglandin E2 (PGE₂) (a), 10 μM 15-deoxy-delta-12,14-prostaglandin J2 (15PGJ₂) (b), 10 μM 12-prostaglandin J2 (12PGJ₂) (c), or 16,16-dimethyl-PGD2 (d), infected at 2 dpf (a–d), and survival (a–d) and caspase-1 activity (e) determined as described in Fig. 2a,b. The sample size for each treatment is 310 in a–d, 30 in e. auf, arbitrary units of fluorescence; NS, not significant; SI, ST infection; *P<0.05; **P<0.01; ***P<0.001 according to log-rank test (in a–d) or ANOVA followed by Tukey multiple range test (e).