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. 2016 Jul 4;7:12044. doi: 10.1038/ncomms12044

Figure 5. COPS5-mediated 4OHT-resistance is dependent on NCoR downregulation.

Figure 5

(a) Western blot analysis of COPS5 and NCoR in MCF7 cells with transient expression of COPS5 and/or NCoR. (b) Quantitative RT–PCR analysis of ligand-regulated ERα target gene expression in parental and 4OHT-resistant MCF7 clones with expression of NCoR. *Significant difference compared with corresponding untreated basal levels (P<0.01, Student's t-test). (c) Western blot analysis of COPS5, NCoR and ERα expression in the 4OHT-resistant MCF7 cells stably transduced with COPS5 shRNA or rescuing COPS5 cDNA. (d) Quantitative RT–PCR analysis of ERα target gene pS2 in the MCF7 cell lines shown in c in response to 4OHT (500 nM, 48 h). *Significant difference compared with the untreated CTL sample (P<0.01, Student's t-test). (e) Western blot analysis of NCoR and ERα expression on knockdown by stable NCoR shRNAs in parental MCF7 cells. (f) Quantitative RT–PCR analysis of ERα target gene pS2 in the MCF7 cell lines shown in e in response to 4OHT (500 nM, 48 h). *Significant difference compared with the untreated CTL sample (P<0.01, Student's t-test). Data are presented as mean+s.e.m. of three biological replicates. CTL, control.