Figure 3. EHMT2 dimethylated H3K9 found in the transgenerational group by RNA-Seq.

(a) Venn diagram of the overlapping significant upregulated or downregulated genes shared among F0H_F0N, F2T_F2N and F2H_F2N (n=6). (b) Clustering of 57 overlapping genes in F0H, F2T and F2H. Red and blue bars represent upregulation and downregulation, respectively (n=6). (c) Validation of the differential expression of euchromatic histone-lysine N-methyltransferase 2 (EHMT2), protein tyrosine kinase 2B (PKT2B) and mitogen-activated protein kinase 9 (MAPK9) by qRT-PCR. Data are presented as the means±s.e.m. *P<0.05 (n=10). (d) H3K9me2 abundance in testicular tissue as revealed by immunostaining. Positive signals are indicated by brownish colouring (n=3).