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. 2016 Jul 4;7:12082. doi: 10.1038/ncomms12082

Figure 1. Neuritic plaques stimulate the phosphorylation of tau.

Figure 1

(a) Brain sections of APPswe;PS1ΔE9 mice (n=7) were detected by antibodies specific to Aβ (6E10), ubiquitin, Microtubule-associated protein 2 (Map2), neurofilament (Smi31, Smi312, and NF-H), and antibodies specific to phosphorylated tau: CP13 and PHF-1, Tau pT231, Tau pS262, Tau pS396, and Tau-pS422. Note the accumulation of phosphorylated tau surrounding the neuritic plaques. Scale bar, 50 μm. (b) Confocal microscopic analysis of Aβ and tau in cortex of APPswe;PS1ΔE9 mice (n=6). Brain sections co-stained with antiserums specific to: Aβ (6E10) and tau (pS262) (upper panel); or Aβ (6E10) and tau (pS422) (lower panel). Scale bar, 50 μm. (c) Confocal microscopic analysis of Ubiquitin and tau in cortex of APP;PS1ΔE9 mice (n=6). Brain sections co-stained with antiserums specific to: Ubiquitin and tau (CP13) (upper panel); or Ubiquitin and PHF-1 (lower panel). Scale bar, 50 μm. (d) Accumulation of phosphorylated tau in dystrophic neurites surrounding the central Aβ core in 6-month-old APPswe;PS1ΔE9 mice (n=9) as detected by antibodies specific to Aβ (6E10) and phosphorylated tau (pS422). Scale bar, 100 μm. (e) Accumulation of phosphorylated tau in dystrophic neurites surrounding the central Aβ core in 12-month-old APPswe;PS1ΔE9 mice (n=11) as detected by antibodies specific to Aβ (6E10) and phosphorylated tau (pS422). Scale bar, 100 μm. (f) No Gallyas positive tau tangle was detected around the Aβ core of neuritic plaques (Congo red, arrows) in APPswe;PS1ΔE9 mice (n=9), even up to 24 months. Scale bar, 25 μm.