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. 2016 Jul 4;7:12082. doi: 10.1038/ncomms12082

Figure 6. Neuritic plaque-dependent acceleration of pathological conversion of tau induced by a mutant tau repeat domain.

Figure 6

(a) Protein blot analysis of tau and APP in brain lysates of 3 month-old female TTA (n=6), tetO-tauRDΔK (n=6), AP (n=3), TTA-AP (n=7), Tau4RΔK (n=5), Tau4RΔK-AP (n=4) and nTG (n=7) mice using antisera K9JA and CT15. (b) Representative brains of 9 month-old female TTA, AP, TTA-AP, Tau4RΔK, Tau4RΔK-AP and nTG mice. Note marked forebrain atrophy in Tau4RΔK mice. (c) Immunostaining of brain sections of 6-month-old female TTA-AP (n=7) and Tau4RΔK-AP mice (n=5) with antibodies 6E10 specific to human Aβ. Scale bars, 200 μm. (d) Immunostaining of 6 month-old female brain sections of Tau4RΔK (n=4), and Tau4RΔK-AP mice (n=4) using antiserum tau-pS422. Scale bar, 50 μm. (e) Aβ plaques (arrows) and abundance of tau aggregates (arrowheads) were observed in Tau4RΔK-AP mice (n=5) at 9 months of age by Thioflavin-T staining. Scale bar, 50 μm. (f) Silver staining to detect Aβ plaques (arrows) and tau tangles (arrowheads) in brain of Tau4RΔK-AP mice (n=5). Scale bar, 50 μm. (g) Immunostaining of brain sections of 9 month-old female Tau4RΔK (n=5), and Tau4RΔK-AP mice (n=4) using tau-pS422. Scale bar, 100 μm. (h) Gallyas-Braak silver staining of brain sections of female Tau4RΔK-AP mice at 6 (top panel, n=4), 9 (middle panel, n=6) and 12 (bottom panel, n=3) months of age (counterstained with fast red). The left panel: sagittal section (Scale bar, 1,000 μm); middle and right panel are hippocampal (HP) and cortical regions (CT), respectively (Scale bar, 25 μm).