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. 2016 Jul 4;214(1):77–88. doi: 10.1083/jcb.201601082

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© 2016 Miyata et al.

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Figure 7. — Ups2–Mdm35 facilitates PS conversion to PE in mitochondria. (A) 4m and 4m UPS2↑MDM35↑ cells were cultured in YPD. Logarithmically growing cells were harvested and analyzed by Western blotting with antibodies against Ups2, Mdm35, and Pgk1. Of note, anti-Ups2 and anti-Mdm35 antibodies could hardly detect endogenous Ups2 and Mdm35 in 4m cells, whereas clear Ups2 and Mdm35 signals were detected in a protein sample of 4m UPS2↑MDM35↑ cells with the respective antibodies. (B and C) PS conversion to PE in 4m and 4m UPS2↑MDM35↑ cells growing in YPD was analyzed as in Fig. 5. The mean values for nine (4m cells) or two (4m UPS2↑MDM35↑ cells) independent experiments and deviations are shown. Closed circle, 4m cells; open square, 4m UPS2↑MDM35↑ cells.