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. 2016 Jul 5;6:29302. doi: 10.1038/srep29302

Figure 1. Expression of p66Shc mRNA and protein in PBMs and correlation analysis.

Figure 1

p66Shc expression in the PBMs of DN patients was determined by real-time PCR analyses (A). Western blot analyses of p66Shc and p-p66Shc protein expression in PBMs (B,C). (D,E) Quantification of average band intensity of Western blots (Control: n = 36; Class IIa: n = 15; Class IIb: n = 18; Class III: n = 17). (F) Bar graph represents the ratio of p-p66Shc to p66Shc expression, as assessed by quantification of relative band intensity. Control: n = 36; Class IIa:n = 15; Class IIb:n = 18;Class III:n = 17. *p < 0.01 versus control; #p < 0.05 versus class IIa; @p < 0.01 versus IIb. Values are shown as the mean ± SD. Correlation analysis of the expression level of p66Shc in PBMs of patients with DN and duration of diabetes (G), triglycerides (H), LDL-C (I), HbA1C (J), blood glucose (K) and SBP (L). Values are means ± E.