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. 2016 Jul 5;6:28508. doi: 10.1038/srep28508

Figure 1. Generation of Lep and Lepr mutant rats with the CRISPR/Cas9 system.

Figure 1

(A) DNA sequences of the targeting sites at Lep and Lepr loci. The sgRNA targeting sequence is shown in black, and the PAM sequence is shown in blue. (B) A male Lep Mutant rat (# 11) showed obese phenotype at age of wk-12 compared to a male WT rat. (C) Representative sequencing results of the mutant alleles in the founders. The gRNA-targeting sequences are underlined and the PAM is highlighted in blue. The deletions are indicated as – whereas the insertions are in red. The number of insertions (+) or deletions (−) are shown to the right of each allele.