Figure 4.

PBISe inhibits AKT/PI3K, MAPK/ERK and STAT3 signaling in human HCC. Four different cell lines were used to demonstrate the late apoptotic process. (A) Skhep1; (B) HepG2; (C) C3A; (D) Huh-7. 1 × 10⁶ cells were treated with increasing conc. of PBISe 2–10 μM for 48 h. Cell lysates were prepared and Western blot analyses were performed. As observed in the figure, pAKT, pERK and pSTAT3 were decreased in a dose dependent manner, suggesting that PBISe has a role not only on PI3K and MAPK but also on STAT3 signaling pathway.