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. 2015 Nov 9;35(26):3465–3475. doi: 10.1038/onc.2015.409

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Copyright © 2016 Macmillan Publishers Limited

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/4.0/

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The migration of DU145 and PC3 cells is promoted by cytoplasmic PML. (a) Schematic representation of PMLI mutant constructs (PMLI WT-HA, PMLI ΔNLS-HA and PML ΔNES-HA) and their protein expression profiles as shown by immunoblotting from DU145 and PC3 whole-cell extracts and using an anti-HA antibody. (b) Immunofluorescence images demonstrating stable expression of PMLI mutants in DU145 and PC3 cells using an anti-HA antibody. Scale bar=10 μm. (c, d) Wound healing assay (Scratch assay) using DU145 and PC3 cells expressing PMLI mutant constructs and the corresponding data quantifying gap closure at time points 0 and 24 h following scratching. Analysis of variance (ANOVA): ****P<0.0001 and ***P=0.0001. Scale bar=200 μm. (e, f) Well cell migration assay using DU145 (left panel) and PC3 cells expressing PMLI mutant constructs and the data relating to the number of migrated cells after 24 h of culture. ANOVA: **P=0.0018 and ANOVA: ***P=0.0002. (g, h) Cell invasion assay using DU145 (left panel) and PC3 cells (right panel) expressing PMLI mutant constructs and the data relating to the number of migrated cells after 24 h of culture. ANOVA: *P=0.0108 and ANOVA: *P=0.0304.