. 2015 Oct 14;29(8):1145–1151. doi: 10.5713/ajas.15.0553

Table 3.

Effects of vitamin D₃ and 25-OH-D₃ on femur mineralization in 1- to 21-d-old broiler chickens

Vitamin D₃ (μg/kg)	25-OH-D₃ (μg/kg)	Weight (g)	Length (cm)	Ash (g)	Ash (%)	Ca (%)	P (%)
0	0	0.56^d	3.43^f	0.17^d	26.64^e	10.30^e	4.92^f
2.5		0.63^cd	3.75^ef	0.20^d	33.89^cd	12.70^cd	6.04^de
5.0		0.78^b	4.34^bcd	0.29^c	37.55^bc	13.01^cd	6.78^cde
10.0		1.01^a	4.69^ab	0.40^ab	39.55^b	14.02^abc	7.13^bc
20.0		1.07^a	4.87^a	0.45^a	43.71^a	15.20^ab	7.88^ab
	1.25	0.69^bc	3.85^def	0.23^cd	33.07^d	11.31^de	5.91^e
	2.5	0.80^b	4.10^cde	0.29^c	36.37^bcd	13.48^bc	6.68^cde
	5.0	0.98^a	4.58^abc	0.38^b	38.64^b	14.52^abc	6.95^cd
	10.0	1.02^a	4.77^ab	0.45^a	44.14^a	15.86^a	8.22^a
SEM		0.03	0.08	0.02	0.81	0.28	0.16
p value
Vitamin D₃	Linear	<0.001	<0.001	<0.001	<0.001	<0.001	<0.001
	Quadratic	0.352	0.268	0.288	0.033	0.318	0.207
25-OH-D₃	Linear	<0.001	<0.001	<0.001	<0.001	<0.001	<0.001
	Quadratic	0.424	0.590	0.457	0.368	0.761	0.936

25-OH-D₃, 25-hydroxycholecalciferol; SEM, standard error of the mean.

^a–f

Means in the same column without a common superscript differ significantly (p<0.05).