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. 2016 Jul 5;6:28965. doi: 10.1038/srep28965

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ClustalW alignment (most appropriate BLOSUM scoring matrix selected by ClustalW) of AAV8, AAV2, and mAAV-EVE1 VP1 coat proteins. Nomenclature and approximate location of secondary structural features are from Govindasamy et al.⁹⁰, except for βA which is from Xie et al.⁹⁵ Beta-strands are indicated by arrows. The position of the lone α-helix is indicated by a purple rectangle. PLA2, phospholipase A2 domain. Catalytic residues of the PLA2 domain are indicated by asterisks. Residues known to form a calcium-binding loop are indicated by hashtags.