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. 2016 Jul 5;6:29135. doi: 10.1038/srep29135

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(A) TBS extract from the Atlantic oyster (Crassostrea gigas) was applied to a mannose-conjugated cellulose column (1.4 × 3.5 cm) equilibrated with TBS. CGL1 that was bound to the column was eluted with TBS containing 100 mM mannose (arrow). ABS: absorbance. (B) an SDS-PAGE pattern under reducing (R) and nonreducing (NR) conditions. Numbers on the left indicate the molecular weights of marker proteins as follows: BSA (66 kDa), ovalbumin (45 kDa), carbonic anhydrase (29 kDa), and cytochrome C (12.5 kDa). M, molecular marker; C, crude extract; and L, lectin.