Figure 6. Illustration of the interactions between mannose and residues at sites A and B of CGL1.
Mannose and residues at site A (panels A–C) and site B (panels D,E) are shown as stick figures. The dotted lines denote hydrogen bonds. A, a stereo view of mannose and the interacting residues at site A. B, β-mannose and the interacting Glu59 at site A with 2Fo-Fc and Fo-Fc electron density maps. C, superposition of anomeric mannoses and interacting Glu59 with the 2Fo-Fc electron density map. D, a stereo view of mannose and the interacting residues at site B. E, mannoses and interacting Glu130 at site B with the 2Fo-Fc electron density map. The 2Fo-Fc and Fo-Fc electron density maps are blue and green and are contoured at 1.5σ and 4σ, respectively.