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. 2016 Jun 24;20(2):122–130. doi: 10.5213/inj.1632552.276

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Copyright © 2016 Korean Continence Society

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 4. — (A) Direct staining of miRNAs in benign prostatic hyperplasia (BPH) tissues, (B) noncancerous surrounding tissues, and (C) cancerous tissues using a QD565-hsv1-miR-H18 and QD565-hsv2-miR-H9 specific beacon detection system. (D) Fluorescence emission of the 2 microRNAs (miRNAs) was higher in prostate cancer tissue and the surrounding noncancerous tissue than in BPH tissue. Staining for the miRNAs was observed in the cytoplasm of cancer cells and in the cells of the surrounding noncancerous tissue, with the epithelial layer staining more strongly than the stroma. Luminal cells, which have direct contact with seminal fluid, showed the strongest staining.