Figure 2.

Binding ability of 10E8-1P and 10E8-4P to 10E8 (A) and 4E10 (B) antibodies. Immunogenic proteins at 5 μg/mL were used to coat plates overnight at 4 °C. The plates were blocked with 2% non-fat milk in PBS, and this was followed by incubation with different samples serially diluted in PBS and incubated at 37 °C for 1 h. Horseradish peroxidase-labeled goat anti-human IgG antibody and 3,3′,5,5′-tetramethylbenzidine were added sequentially. The absorbance at 450 nm (A₄₅₀) was determined. Each sample was tested in triplicate. The results shown are a representative set of data from two independent experiments. phosphate-buffered saline, PBS.