Figure 1.
Schematic representation of mIL-12-expressing HSV (M002). Line 1 illustrates the HSV-1 (F) Δ305 genome, which contains a 700 bp deletion within the tk gene, as indicated by the Δ symbol. UL and US represent the unique long and unique short sequences, respectively. The inverted repeat sequences are indicated by a, b, and c, with subscripts n and m representing variable numbers of a sequences. a1 and as represent the a sequences flanking the UL and US terminal repeats. Line 2 shows the sequence arrangement of the recombinant HSV R3659. The BstEII–StuI fragment within the γ134.5 gene was replaced by the chimeric α27-tk gene in the inverted sequences ab (shown above) and b′a′ (not shown) flanking the UL sequence. Line 3 shows the sequence arrangements of the relevant regions in the recombinant mIL-12-expressing HSV M001 (tk−) or M002 (tk+). NcoI restriction sites are indicated. (Copyright [2000] National Academy of Sciences, U.S.A. Reproduced with permission from PNAS [Parker et al.12]).