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Immunocompetent BALB/c mice were injected subcutaneously with 1 × 105 neuro-2A cells. When tumors attained 200 mm3 in volume, they were injected with 50 liters of saline or 5 × 107 PFU of HSV R3659 or M002. Tumor volumes were measured every 3 days, and the average percentage increase in volume was calculated relative to the treatment date.
