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. 2016 Jul 4;16:379. doi: 10.1186/s12885-016-2470-3

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — SIAH1 silencing inhibits cell migration of breast cancer cells. (a) Western blot assays for SIAH1 knock-down in MDA-MB-231 cells using shSIAH1.2 interfering sequence. Scramble sequence was transfected as negative control. GAPDH was used as internal loading control. (b) Densitometric analysis of immunodetected bands in panel A. (c) Quantification of scratch/wound healing assays in non-transfected control, scramble transfected and SIAH1-deficient cells. Data represents the mean of three independent assays ± SD. (*p < 0.05)