Table 2.
Quantitative trait loci for stomatal traits and yield
| Trait/experiment | QTL symbol | Chr arm | Closest marker | Peak (cM) | LOD | AE | R2 (%) |
|---|---|---|---|---|---|---|---|
| Lameroo | |||||||
| SD | QSD.afl-7A | 7A1L | wsnp_Ra_rep_c104968_8898575 | 79.1 | 5.6 | 1.4 | 10 |
| APL | QAPL. afl-2B | 2BS | wsnp_Ra_c18396_27453775 | 42.41 | 5.4 | 1.0 | 10 |
| QAPL. afl-7A | 7A1L | BobWhite_rep_c49790_351 | 85.2 | 5.2 | −1.0 | 5 | |
| GCL | QGCL. afl-7A | 7A1L | CAP8_c3496_118 | 83.7 | 6.5 | −1.2 | 14 |
| QGCL. afl-3B | 3BL | adli16 | 122.18 | 4.3 | −0.2 | 9 | |
| GCA | QGCA. afl-1B | 1BL | wPt.0944 | 158.44 | 3.6 | 14.9 | 8 |
| QGCA. afl-4B | 4BL | BS00023024_51 | 86.2 | 3.5 | 13.8 | 6 | |
| QGCA. afl-5D | 5DL | RAC875_c41914_613 | 188.12 | 3.5 | 14.3 | 8 | |
| YLD | QYLD. afl-4A | 4AS | BS00022177_51 | 14.9 | 9.0 | 0.17 | 23 |
| Roseworthy | |||||||
| SD | QSD.afr-4A | 4AS | BS00011060_51 | 36.0 | 3.6 | −0.6 | 8 |
| QSD. afr-5A | 5AS | TA003745.0632 | 7.1 | 6.4 | 0.8 | 18 | |
| SI | QSI. afr-2B | 2BL | Kukri_c657_1139 | 84.2 | 3.1 | 0.5 | 17 |
| QSI. afr-7B | 7BL | BobWhite_c33540_69 | 64.4 | 3.1 | 0.5 | 15 | |
| APL | QAPL. afr-2B | 2BL | wPt.3378 | 94.75 | 2.9 | −0.7 | 9 |
| QAPL. afr-7A | 7A1L | BobWhite_c1201_384 | 98.73 | 2.8 | 0.7 | 9 | |
| APA | QAPA. afr-7A | 7A1L | BobWhite_c1201_384 | 98.73 | 4.5 | −7.6 | 14 |
| GCL | QGCL. afr-1A | 1AS | wsnp_Ku_c34659_43981982 | 35.02 | 4.1 | 0.9 | 13 |
| QGCL. afr-7A | 7A1L | BobWhite_c1201_384 | 95.5 | 3.5 | 0.1 | 8 | |
| GCA | QGCA. afr-5A | 5AL | Kukri_c28080_887 | 109.9 | 3.9 | −7.2 | 6 |
| YLD | QYLD. afr-7A | 7A1L | BobWhite_c15497_199 | 84.43 | 4.53 | 0.24 | 16 |
| QYLD. afr-7B | 7BS | RAC875_c7123_1703 | 46.45 | 3.0 | 0.39 | 9 | |
| WW | |||||||
| SD | QSD.atw-3A | 3A2L | BobWhite_c43681_334 | 90.9 | 6.7 | 0.7 | 26 |
| QSD.atw-4B | 4BS | tplb0061a20_153 | 67.3 | 7.8 | 0.7 | 28 | |
| QSD.atw-7A | 7A1S | Excalibur_c15260_94 | 51.2 | 4.2 | 0.5 | 14 | |
| SI | QSI.atw-3D | 3D2S | Excalibur_c3510_1888 | 19.67 | 4.5 | −6.3 | 23 |
| APL | QAPL.atw-1A | 1AS | Tdurum_contig9199_714 | 39.15 | 4.9 | 1.3 | 13 |
| QAPL.atw-4B | 4BS | BS00037094_51 | 21.7 | 5.9 | −0.9 | 19 | |
| APW | QAPW.atw-1B | 1BL | barc0207 | 116.56 | 6.0 | 0.9 | 22 |
| QAPW.atw-7A | 7A1L | BobWhite_c23146_84 | 76.0 | 6.6 | −0.5 | 22 | |
| QAPW.atw-2D | 2DS | Ex_c10377_845 | 60.85 | 12.6 | 0.7 | 22 | |
| APA | QAPA.atw-2D | 2DS | Ex_c10377_845 | 60.85 | 14.6 | 31.7 | 53 |
| QAPA.atw-7A | 7A1L | BobWhite_c23146_84 | 76.0 | 5.8 | −18.5 | 18 | |
| GCL | QGCL.atw-1B | 1BL | barc0207 | 116.56 | 5.7 | 1.5 | 23 |
| QGCL.atw-7D | 7DL | BS00067285_51 | 113.0 | 4.0 | 0.9 | 15 | |
| GCW | QGCW.atw-2D | 2DS | Ex_c10377_845 | 60.85 | 4.0 | 0.5 | 13 |
| QGCW.atw-6A | 6AS | BS00061749_51 | 21.97 | 4.1 | 0.5 | 14 | |
| GCA | QGCA.atw-4A | 4AL | BS00022395_51 | 133.2 | 3.3 | 20.0 | 12 |
| QGCA.atw-4B | 4BS | Kukri_c26488_139 | 18.8 | 5.0 | 27.0 | 19 | |
| D | |||||||
| SD | QSD.atd-5B | 5BS | Excalibur_c29975_333 | 28.6 | 5.2 | 0.9 | 19 |
| SI | QSI.atd-5B | 5BS | Excalibur_c17904_437 | 34.3 | 4.8 | 6.1 | 19 |
| QSI.atd-6D | 6DL | RFL_Contig1159_2123 | 106.22 | 5.2 | 6.2 | 18 | |
| APL | QAPL.atd-2B | 2BL | Excalibur_c15242_306 | 87.39 | 3.0 | −1.0 | 14 |
| QAPL.atd-4B | 4BS | BS00037094_51 | 22.8 | 4.0 | −0.8 | 11 | |
| QAPL.atd-7A | 7A1S | Kukri_c4700_1266 | 1.7 | 3.7 | −0.8 | 10 | |
| QAPL.atd-7D | 7DL | BS00067285_51 | 129.3 | 5.0 | −0.4 | 17 | |
| GCL | QGCL.atd-1B | 1BL | wPt.0944 | 158.44 | 3.4 | 1.4 | 15 |
| QGCL.atd-4B | 4BS | Excalibur_c7581_1266 | 24.6 | 6.0 | −1.3 | 26 | |
| QGCL.atd-7A | 7A1S | BS00081098_51 | 4.6 | 6.0 | −1.4 | 26 | |
| GCA | QGCA.atd-1B | 1BL | wsnp_Ra_c21132_30487331 | 112.91 | 4.0 | 17.4 | 17 |
| QGCA.atd-5B | 5BS | Excalibur_c29975_333 | 28.5 | 6.5 | −25.0 | 32 | |
Chromosomal location, closest linked marker, position of the QTL peak, LOD score, estimated additive effects (AE, in trait unit) and percentage of phenotypic variance (R2) for QTL assessed in RAK875/Kukri DH population in the field (Lameroo and Roseworthy), and under well-watered (WW) and drought (D) glasshouse conditions. A positive AE value means the trait increased is due to RAC875 allele at the QTL. APA: aperture area, APL: aperture length, APW: aperture width, GCA: guard cell area, GCL: guard cell length, GCW: guard cell width, SD: stomatal density, SI: stomatal index, YLD: yield