Fig. 5.

Azacytidine increases Treg frequency in the thymus on day +35. Balb/cJ mice were injected i.v. with 10.10⁶ bone marrow cells and 70.10⁶ splenocytes from B10.D2 donor mice after lethal irradiation. Mice were then given (or not) azacytidine (AZA, 2 mg/kg) administered subcutaneously every 48 h from day +10 to day +30. On day +35, thymus from control (n = 6) and AZA-treated mice (n = 6) were collected. a, b FACS analyses showing the frequencies of double negative CD4/CD8 cells (DN), double-positive CD4/CD8 cells (DP), single-positive CD4 cells (CD4 SP), and single-positive CD8 cells (CD8 SP) populations in the thymus (a) and the frequency of regulatory T cells among CD4 single-positive T cells (b). c Representative graphs showing CD4⁺ T cells discriminated into conventional (CD4⁺Foxp3⁻) and regulatory T cells (CD4⁺Foxp3⁺) in control and AZA-treated mice. d Histological evaluation (Hematoxylin-eosin, 100×) of thymic architecture in representative control and AZA-treated mice. Results are expressed as median, 25th and 75th percentiles of the distribution (boxes), and whiskers extending to the 5th and 95th percentiles. *P < 0.05; **P < 0.01