Table 1.
Strongest positive and negative correlation between the most expressed messenger RNA (top upregulated and downregulated genes) and miR-155/miR-21/miR-4459 lung expression
| Abbreviation | Gene name | Fold-change | r (miR-155) | r (miR-21) | r (miR-4459) |
|---|---|---|---|---|---|
| MMP1 | Matrix metallopeptidase 1 (interstitial collagenase) | 14.1 | 0.13 | 0.24 | -0.52 |
| MMP7 | Matrix metallopeptidase 7 (matrilysin, uterine) | 12.0 | 0.57 | 0.54 | -0.85 |
| KRT15 | Keratin 15 | 7.1 | 0.27 | 0.40 | -0.51 |
| S100A2 | S100 calcium binding protein A2 | 6.6 | 0.29 | 0.45 | -0.57 |
| PDIA3 | Protein disulfide isomerase family A, member 3 | 6.2 | 0.67 | 0.52 | -0.76 |
| CDH3 | Cadherin 3, type 1, P-cadherin (placental) | 5.6 | 0.60 | 0.60 | -0.82 |
| IL13RA2 | Interleukin 13 receptor, alpha 2 | 5.6 | 0.75 | 0.81 | -0.78 |
| IGHM | Immunoglobulin heavy constant mu | 5.5 | 0.67 | 0.52 | -0.63 |
| SPP1 | Secreted phosphoprotein 1 | 5.1 | 0.69 | 0.55 | -0.74 |
| GPR87 | G protein-coupled receptor 87 | 4.7 | 0.29 | 0.32 | -0.45 |
| RRM2 | Ribonucleotide reductase M2 | 4.7 | 0.72 | 0.59 | -0.57 |
| CTSE | Cathepsin E | 4.4 | 0.66 | 0.66 | -0.91 |
| AADAC | Arylacetamide deacetylase | 4.4 | 0.60 | 0.48 | -0.76 |
| CXCL6 | Chemokine (C-X-C motif) ligand 6 (granulocyte chemotactic protein 2) | 4.3 | 0.34 | 0.34 | -0.30 |
| CR2 | Complement component (3d/Epstein Barr virus) receptor 2 | 4.2 | 0.61 | 0.37 | -0.42 |
| TYMS | Thymidylate synthetase | 4.2 | 0.72 | 0.58 | -0.75 |
| CXCL13 | Chemokine (C-X-C motif) ligand 13 | 4.1 | 0.77 | 0.59 | -0.48 |
| SCG5 | Secretogranin V (7B2 protein) | 4.1 | 0.76 | 0.70 | -0.70 |
| MEOX1 | Mesenchyme homeobox 1 | 3.9 | 0.59 | 0.52 | -0.51 |
| TOP2A | Topoisomerase (DNA) II alpha 170 kDa | 3.7 | 0.68 | 0.62 | -0.64 |
| POSTN | Periostin, osteoblast specific factor | 3.7 | 0.74 | 0.70 | -0.74 |
| Pearson’s correlation >0.5 or < -0.5 | total | n = 16 | n = 14 | n = 17 | |
| IL6 | Interleukin 6 (interferon, beta 2) | -45.2 | -0.50 | -0.44 | 0.54 |
| SERPINE1 | Plasminogen activator inhibitor type 1, member 1 | -21.9 | -0.45 | -0.36 | 0.60 |
| FOSB | FBJ murine osteosarcoma viral oncogene homolog B | -18.4 | -0.69 | -0.66 | 0.73 |
| MT1M | Metallothionein 1 M | -15.8 | -0.50 | -0.41 | 0.54 |
| DDX3Y | DEAD (Asp-Glu-Ala-Asp) box polypeptide 3, Y-linked | -14.3 | -0.17 | -0.51 | 0.33 |
| RND1 | Rho family GTPase 1 | -13.6 | -0.46 | -0.37 | 0.40 |
| IL8 | Interleukin 8 | -12.8 | -0.40 | -0.26 | 0.37 |
| NXF3 | Nuclear RNA export factor 3 | -12.0 | -0.80 | -0.75 | 0.89 |
| CSF3 | Colony stimulating factor 3 (granulocyte) | -11.8 | -0.50 | -0.34 | 0.48 |
| CCL2 | Chemokine (C-C motif) ligand 2 | -11.6 | -0.47 | -0.36 | 0.54 |
| PTX3 | Pentraxin 3, long | -11.4 | -0.15 | -0.02 | 0.27 |
| IL12A | Interleukin 12A | -11.1 | -0.70 | -0.64 | 0.85 |
| CCL20 | Chemokine (C-C motif) ligand 20 | -9.5 | -0.25 | -0.17 | 0.15 |
| GADD45B | Growth arrest and DNA-damage-inducible, beta | -9.5 | -0.50 | -0.42 | 0.54 |
| ESM1 | Endothelial cell-specific molecule 1 | -8.6 | -0.52 | -0.36 | 0.48 |
| CXCL2 | Chemokine (C-X-C motif) ligand 2 | -8.5 | -0.40 | -0.44 | 0.46 |
| SLC6A4 | Solute carrier family 6 (neurotransmitter transporter, serotonin), member 4 | -8.4 | -0.82 | -0.87 | 0.72 |
| SLCO4A1 | Solute carrier organic anion transporter family, member 4A1 | -8.3 | -0.62 | -0.50 | 0.64 |
| KDM5D | Lysine (K)-specific demethylase 5D | -8.2 | -0.15 | -0.48 | 0.37 |
| S100A12 | S100 calcium binding protein A12 | -8.2 | -0.45 | -0.33 | 0.53 |
| HAS2 | Hyaluronan synthase 2 | -8.2 | -0.29 | -0.27 | 0.30 |
| Pearson’s correlation >0.5 or < –0.5 | total | n = 9 | n = 6 | n = 10 |
In sequence: gene symbol, gene name, average fold-change expression in SSc-ILD compared to healthy controls, r values for correlation between each gene and specific miRNA