Table 2.
Stages of Camelina seed development and RNA-Seq data sets
| Cs-14 (10–15 DAF) | Cs-21 (16–21 DAF) | |||||||
|---|---|---|---|---|---|---|---|---|
| Bio Rep 1 | Bio Rep 2 | Bio Rep 3 | Mean ± SD | Bio Rep 1 | Bio Rep 2 | Bio Rep 3 | Mean ± SD | |
| Number of sequence reads | 53.0 M | 54.8 M | 78.9 M | 62.23 ± 14.46 | 62.0 M | 61.7 M | 46.3 M | 56.6 ± 8.9 |
| Number of reads after trimming | 52.0 M | 53.7 M | 72.5 M | 59.40 ± 11.37 | 60.7 M | 60.5 M | 45.4 M | 55.5 ± 8.7 |
| Reads mapped in pairs | 44.8 M | 45.6 M | 67.3 M | 52.57 ± 12.76 | 50.7 M | 51.1 M | 38.6 M | 46.8 ± 7.1 |
| Reads mapped in broken pairs | 5.9 M | 6.7 M | 8.4 M | 7.00 ± 1.27 | 8.4 M | 7.9 M | 5.8 M | 7.36 ± 1.3 |
| Reads not mapped | 210,281 | 241,410 | 372,878 | 274,856 ± 86,304 | 319,246 | 286,780 | 184,244 | 263,416 ± 70,466 |
Shown are the two stages of Camelina seed development used for RNA-Seq. The number of processed reads obtained from three biological replicates at different levels of data analysis of RNA sequencing (in millions M)
DAF days after flowering; SD standard deviation