Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Jul 4;9:384. doi: 10.1186/s13071-016-1662-7

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

PMC Copyright notice

Fig. 1 — PCR identification of T653K and M1 genes and recombinant plasmids pFastBac-T653K and pFastBac-M1 digested. The T. spiralis T653K gene was PCR-amplified from cDNA synthesized using a Prime Script 1^st Strain cDNA Synthesis Kit using total RNA extracted from T. spiralis Korean isotype (a). Influenza M1 gene was PCR amplified fromtotal RNA extracted from influenza virus (A/PR/8/34) (c). The T. spiralis T653K gene and influenza M1 gene were cloned into pFastBac with EcoRI/XhoI and SamI/XbaI enzymes, respectively, resulting in T653K plasmid (b) and M1 plasmid (d). Marker: DNA marker; size of T653K: 1,239 bp; size of M1: 1,027 bp