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. 2016 Jul 4;9:384. doi: 10.1186/s13071-016-1662-7

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Characterization of virus-like particles (VLPs). Electron microscopy and VLP size determination. Negative staining of VLPs was performed followed by transmission electron microscopy (TEM). The size is between 40 and 120 nm (a). Western blot analysis. VLPs (20, 10, 5 μg) were loaded for SDS-PAGE. Polyclonal mouse anti-T. spiralis antibody was used to probe T653k protein and anti-M1 monoclonal antibody was used to determine M1 protein (b)