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. 2016 Jul 4;9:384. doi: 10.1186/s13071-016-1662-7

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Experimental schedule and T. spiralis-specific IgG subtype (IgG1 and IgG2a) responses upon immunization. Mice were immunized twice with VLPs as indicated with a 4-week interval. Challenge infection was performed at week 4 after last immunization (a). Enzyme-linked immunosorbent assay (ELISA) plates were coated with T. spiralis antigen, as indicated in Methods. T. spiralis-specific IgG, IgG1 and IgG2a antibody responses in the sera were determined after prime and boost. Significant differences were found between group VLPs alone and group VLP with cholera toxin (CT) (b, c and d, *P < 0.05)