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. 2016 Apr 29;8(2):84–92. doi: 10.1038/ijos.2015.57

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SIRT6 knock out mice. (a, d) Body weight was determined in ~9–11 mice for each group. (b) X-ray image. (c) Alizarin red S- and Alcian blue staining for skeletons. (e, f) Osteocalcin and TRAP5b concentration in blood was analyzed using enzyme-linked immunosorbent assay (ELISA) with ~11–12 mice in each group. *P<0.05. Het, heterozygote; KO, knock out; OCN, osteocalcin; WT, wild type.