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. 2016 Apr 29;8(2):84–92. doi: 10.1038/ijos.2015.57

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Histological examination. (a) Haematoxylin and eosin staining of the femur nearby the metaphysic area. (b) Van Gieson (red) and Von Kossa (black) staining for the mineralization of bone tissue. (c) Double-labelling analysis for bone formation at the femoral mid shaft. The red line is marked using xylenol orange, while the green line is marked by calcein. H&E, haematoxylin and eosin; KO, knock out; WT, wild type.