Figure 6.
Day 21 of cell culture for osteoblastogenesis. (a) Expression of ColIα1-GFP, xylenol orange fluorescence for calcification and calcific nodules condition. (b, c) Alkaline phosphatase staining and Von Kossa staining. ALP, alkaline phosphatase; DAPI, 4′,6-diamidino-2-phenylindole; GFP, green fluorescent protein; KO, knock out; WT, wild type; XO, xylenol orange.