FIG. 3.

Defects in the expression of mature mDA neuron markers in Ldb1 mutant embryos at E14.5. (A–D, A′–D′) Coronal brain sections were stained with anti-TH, -Pitx3, -Nurr1, and -GABA antibodies. Staining of TH, Pitx3, and Nurr1 was missing or greatly reduced in Ldb1 mutant embryos (A′, B′, C′) compared with the controls (A, B, C). No change in GABA staining was detected in Ldb1 mutant (D′) compared with the control (D). (E–H, E′–H′) Similar results were shown by whole-mount immunostaining of brains from wild-type (E–H) and Ldb1 mutant (E′–H′) embryos. mDA, midbrain dopaminergic.