FIG. 3.

Cell killing properties of A9.min-rGel. (A) LNCaP, (B) PC3-PSMA, and (C) PC3 cells were incubated with increasing concentrations of A9.min-rGel or a nonfunctional aptamer conjugate control C36-rGel. LNCaP and PC3-PSMA cells both express PSMA. PC3 cells are a PSMA-negative cell line. Assays were performed in growth media containing 10% FBS supplemented with 1 mg/mL ssDNA as a nonspecific blocking agent. Conjugates were added to cells at increasing concentrations and incubated for 5 days after which the fraction of viable cells remaining was determined. The IC50 values for each curve are reported in Table 1.