FIG. 3.

Regulation of PfOAT activity by glutathione. (A) PfOAT was incubated with different concentrations of GSSG for 10 min at 37°C and (B) with different concentrations of GSH for 5 min at 37°C. (C) Time-dependent regulation of PfOAT with 5 mM GSSG and (D) 5 mM GSH. The samples were incubated at 37°C and aliquots were taken every 2 min to monitor the activity. Each value is a mean value from three independent determinations each including 6–8 measurements. Data are represented as mean±standard deviation. GSH, reduced glutathione; GSSG, oxidized glutathione disulfide; OAT, ornithine δ-aminotransferase.