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Enzymatic deglutathionylation of (A) PfTPx1 and (B) PfPK by P. falciparum redoxins. PfTPx1 and PfPK were incubated with 10 mM GSSG over night at 23°C, and excess GSSG was removed by gel filtration chromatography. Glutathionylated PfTPx1 (4.5 μM) and PfPK (9 μM) were incubated with different concentrations of reduced PfTrx1, PfGrx1, and/or PfPlrx for 10 min at 37°C.
